Boster Immunoleader

ELISA/assay

Human DAN/NBL1 PicoKine ELISA Kit

EK0746

1 x96 well plate

369 USD

Human DAN/NBL1 PicoKine ELISA Kit

EK0746

369 USD

Sandwich ELISA kit for Quantitative Detection of Human DAN/NBL1

NSO, A17-D181

1 x96 well plate

125pg/ml-8000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 1257; Standard deviation: 72.90; CV(%): 5.8 Sample 2: n=16; Mean(pg/ml): 3204; Standard deviation: 189.03; CV(%): 5.9 Sample 3: n=16; Mean(pg/ml): 5394; Standard deviation: 334.42; CV(%): 6.2

Sample 1: n=24; Mean(pg/ml): 1020; Standard deviation: 65.28; CV(%): 6.4 Sample 2: n=24; Mean(pg/ml): 3562; Standard deviation: 235.09; CV(%): 6.6 Sample 3: n=24; Mean(pg/ml): 4975; Standard deviation: 353.22; CV(%): 7.1

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human DAN in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human DAN antibody 1 ST0000-10 Lyophilized recombinant human DAN standard 10ng/tube×2 AR1107 Biotinylated anti- human DAN antibody 130μl(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

NBL1

Neuroblastoma suppressor of tumorigenicity 1

neuroblastoma 1, DAN family BMP antagonist

D1S1733E DAN DAN domain family member 1 DAND 1 DAND1 Differential screening selected gene aberrant in neuroblastoma MGC8972 NB NBL 1 Neuroblastoma candidate region suppression of tumorigenicity 1 Neuroblastoma suppression of tumorigenicity 1 Neuroblastoma suppressor of tumorigenicity 1 NO 3 NO3 Zinc finger protein DAN

P41271