Boster Immunoleader

ELISA/assay

Rat CXCL1 PicoKine ELISA Kit

EK0724

1 x96 well plate

400 USD

Rat CXCL1 PicoKine ELISA Kit

EK0724

400 USD

Sandwich ELISA kit for Quantitative Detection of Rat CXCL1

E.coli, A25-K96

1 x96 well plate

15.6pg/ml-1000pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 75.4; Standard deviation: 4.1; CV(%): 5.4 Sample 2: n=16; Mean(pg/ml): 221; Standard deviation: 13.7; CV(%): 6.2 Sample 3: n=16; Mean(pg/ml): 546; Standard deviation: 28.4; CV(%): 5.2

Sample 1: n=24; Mean(pg/ml): 98.4; Standard deviation: 6; CV(%): 6.1 Sample 2: n=24; Mean(pg/ml): 285; Standard deviation: 20.52; CV(%): 7.2 Sample 3: n=24; Mean(pg/ml): 534; Standard deviation: 31.5; CV(%): 5.9

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat CXCL1 in cell culture supernates, serum and plasma(EDTA).

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat CXCL1 antibody 1 ST0000-10 Lyophilized recombinant rat CXCL1 standard 10ng/tubex2 AR1107 Biotinylated anti- rat CXCL1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Chemokine(C-X-C motif) ligand 1(CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3(NAP-3) and melanoma growth stimulating activity, alpha(MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.The standard product used in this kit is recombinant rat CXCL1, consisting of 72 amino acids with the molecular mass of 7.8KDa.

CXCL1

C-X-C motif chemokine 1

chemokine (C-X-C motif) ligand 1

C-X-C motif chemokine 1 chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) fibroblast secretory protein FSP GRO GRO protein, alpha GRO-alpha(1-73) GRO1 GRO1 oncogene GRO1 oncogene (melanoma growth stimulating activity, alpha) GRO1 oncogene (melanoma growth-stimulating activity) GROa growth-regulated alpha protein Keratinocyte-derived chemokine, mouse, homolog of Melanoma growth stimulatory activity melanoma growth stimulatory activity alpha MGSA MGSA a MGSA alpha NAP 3 Neutrophil-activating protein 3 SCYB1 Small inducible cytokine subfamily B, member 1

P14095