Boster Immunoleader

ELISA/assay

Mouse CTLA4 PicoKine ELISA Kit

EK0717

1 x96 well plate

369 USD

Mouse CTLA4 PicoKine ELISA Kit

EK0717

369 USD

Sandwich ELISA kit for Quantitative Detection of Mouse CTLA4

NSO, A37-F162

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 1.42; Standard deviation: 0.051; CV(%): 3.6 Sample 2: n=16; Mean(ng/ml): 3.53; Standard deviation: 0.138; CV(%): 3.9 Sample 3: n=16; Mean(ng/ml): 6.82; Standard deviation: 0.286; CV(%): 4.2

Sample 1: n=24; Mean(ng/ml): 1.47; Standard deviation: 0.063; CV(%): 4.3 Sample 2: n=24; Mean(ng/ml): 3.71; Standard deviation: 0.171; CV(%): 4.6 Sample 3: n=24; Mean(ng/ml): 6.87; Standard deviation: 0.426; CV(%): 6.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse CTLA4 in cell culture supernates, serum and plasma(heparin).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse CTLA4 antibody 1 ST0000-10 Lyophilized recombinant mouse CTLA4 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse CTLA4 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

CTLA-4(Cytotoxic T-Lymphocyte Antigen 4), also known as CD152(Cluster of differentiation 152), is a protein receptor that down regulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.

CTLA4

Cytotoxic T-lymphocyte protein 4

cytotoxic T-lymphocyte-associated protein 4

Activation B7-1 antigen B7 BB1 CD80 CD80_HUMAN CTLA-4 counter-receptor B7.1 T-lymphocyte activation antigen CD80

P09793