Boster Immunoleader

ELISA/assay

Rat CD86/B7-2 PicoKine ELISA Kit

EK0712

1 x96 well plate

369 USD

Rat CD86/B7-2 PicoKine ELISA Kit

EK0712

369 USD

Sandwich ELISA kit for Quantitative Detection of Rat CD86/B7-2

NSO, V29-I250

1 x96 well plate

62.5pg/ml-4000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 0.21; Standard deviation: 0.010; CV(%): 4.8 Sample 2: n=16; Mean(ng/ml): 1.44; Standard deviation: 0.102; CV(%): 7.1 Sample 3: n=16; Mean(ng/ml): 2.59; Standard deviation: 0.161; CV(%): 6.2

Sample 1: n=24; Mean(ng/ml): 0.23; Standard deviation: 0.013; CV(%): 5.8 Sample 2: n=24; Mean(ng/ml): 1.47; Standard deviation: 0.118; CV(%): 8.0 Sample 3: n=24; Mean(ng/ml): 2.46; Standard deviation: 0.182; CV(%): 7.4

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat CD86 in cell culture supernates and serum.

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat CD86/B7-2 antibody 1 ST0000-10 Lyophilized recombinant rat CD86/B7-2 standard 10ng/tubex2 AR1107 Biotinylated anti- rat CD86/B7-2 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Cluster of Differentiation 86(also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

CD86

T-lymphocyte activation antigen CD86

CD86 molecule

Activation B7 2 antigen B7 2 B70 B72 BU63 CD28LG2 CD86 antigen (CD28 antigen ligand 2 B7 2 antigen) CD86 molecule CTLA 4 counter receptor B7.2 FUN 1 FUN1 T lymphocyte activation antigen CD86 precursor

Q62810