Boster Immunoleader

ELISA/assay

Human B7-1/CD80 PicoKine ELISA Kit

EK0707

1 x96 well plate

369 USD

Human B7-1/CD80 PicoKine ELISA Kit

EK0707

369 USD

Sandwich ELISA kit for Quantitative Detection of Human B7-1/CD80

NSO, V35-N242

1 x96 well plate

62.5pg/ml-4000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 0.22; Standard deviation: 0.013; CV(%): 5.7 Sample 2: n=16; Mean(ng/ml): 1.47; Standard deviation: 0.090; CV(%): 6.1 Sample 3: n=16; Mean(ng/ml): 2.34; Standard deviation: 0.185; CV(%): 7.9

Sample 1: n=24; Mean(ng/ml): 0.27; Standard deviation: 0.018; CV(%): 6.8 Sample 2: n=24; Mean(ng/ml): 1.56; Standard deviation: 0.115; CV(%): 7.4 Sample 3: n=24; Mean(ng/ml): 2.58; Standard deviation: 0.214; CV(%): 8.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human CD80 in cell culture supernates and serum.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human B7-1/CD80 antibody 1 ST0000-10 Lyophilized recombinant human B7-1/CD80 standard 10ng/tubex2 AR1107 Biotinylated anti- human B7-1/CD80 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

CD80

T-lymphocyte activation antigen CD80

CD80 molecule

Activation B7-1 antigen B7 BB1 CD80 CD80_HUMAN CTLA-4 counter-receptor B7.1 T-lymphocyte activation antigen CD80

P33681