Boster Immunoleader

ELISA/assay

Mouse CD14 PicoKine ELISA Kit

EK0695

1 x96 well plate

369 USD

Mouse CD14 PicoKine ELISA Kit

EK0695

369 USD

Sandwich ELISA kit for Quantitative Detection of Mouse CD14

NSO, A18-P345

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 0.98; Standard deviation: 0.038; CV(%): 3.9 Sample 2: n=16; Mean(ng/ml): 3.11; Standard deviation: 0.162; CV(%): 5.2 Sample 3: n=16; Mean(ng/ml): 6.23; Standard deviation: 0.355; CV(%): 5.7

Sample 1: n=24; Mean(ng/ml): 1.03; Standard deviation: 0.081; CV(%): 7.9 Sample 2: n=24; Mean(ng/ml): 3.47; Standard deviation: 0.249; CV(%): 7.2 Sample 3: n=24; Mean(ng/ml): 6.72; Standard deviation: 0.423; CV(%): 6.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse CD14 in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse CD14 antibody 1 ST0000-10 Lyophilized mouse CD14 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse CD14 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein(mCD14) and a monocyte or liver-derived soluble serum protein(sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor(along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide(LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein(LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

CD14

Monocyte differentiation antigen CD14

CD14 molecule

CD 14 CD14 antigen CD14 molecule Monocyte differentiation antigen CD14 Myeloid cell-specific leucine-rich glycoprotein

P10810