Boster Immunoleader

ELISA/assay

Human Cystatin C PicoKine ELISA Kit

EK0678

1 x96 well plate

400 USD

Human Cystatin C PicoKine ELISA Kit

EK0678

400 USD

Sandwich ELISA kit for Quantitative Detection of Human Cystatin C

NSO, M1-A146

1 x96 well plate

312pg/ml-20,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 2.68; Standard deviation: 0.115; CV(%): 4.3 Sample 2: n=16; Mean(ng/ml): 6.13; Standard deviation: 0.288; CV(%): 4.7 Sample 3: n=16; Mean(ng/ml): 12.83; Standard deviation: 0.744; CV(%): 5.8

Sample 1: n=24; Mean(ng/ml): 2.87; Standard deviation: 0.152; CV(%): 5.3 Sample 2: n=24; Mean(ng/ml): 6.24; Standard deviation: 0.356; CV(%): 5.7 Sample 3: n=24; Mean(ng/ml): 13.21; Standard deviation: 0.845; CV(%): 6.4

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Cystatin C in cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine and human milk.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Cystatin C antibody 1 ST0000-20 Lyophilized recombinant human Cystatin C standard 20ng/tubex2 AR1107 Biotinylated anti- human Cystatin C antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Cystatin C or cystatin 3(formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid(CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight(approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus(cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2(enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3(it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4

CST3

Cystatin-C

cystatin C

ARMD11 bA218C14.4 (cystatin C) Cst3 Cystatin-3 Cystatin-C CYTC_HUMAN Gamma-trace Neuroendocrine basic polypeptide Post-gamma-globulin

P01034