Boster Immunoleader

ELISA/assay

Human Cathepsin B PicoKine ELISA Kit

EK0670

1 x96 well plate

400 USD

Human Cathepsin B PicoKine ELISA Kit

EK0670

400 USD

Sandwich ELISA kit for Quantitative Detection of Human Cathepsin B

NSO, R18-I339

1 x96 well plate

156pg/ml-10,000pg/ml

<5pg/ml

Sample 1: n=16; Mean(pg/ml): 1350; Standard deviation: 68.9; CV(%): 5.1 Sample 2: n=16; Mean(pg/ml): 3080; Standard deviation: 150.9; CV(%): 4.9 Sample 3: n=16; Mean(pg/ml): 6170; Standard deviation: 357.9; CV(%): 5.8

Sample 1: n=24; Mean(pg/ml): 1452; Standard deviation: 113.3; CV(%): 7.8 Sample 2: n=24; Mean(pg/ml): 3209; Standard deviation: 256.7; CV(%): 8 Sample 3: n=24; Mean(pg/ml): 6124; Standard deviation: 520.5; CV(%): 8.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Cathepsin B in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Cathepsin B antibody 1 ST0000-10 Lyophilized recombinant human Cathepsin B standard 10ng/tubex2 AR1107 Biotinylated anti- human Cathepsin B antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

CTSB

Cathepsin B

cathepsin B

aAmyloid precursor protein secretase APP secretase APPS CATB_HUMAN Cathepsin B heavy chain Cathepsin B1 CPSB CTSB Cysteine protease

P07858