Boster Immunoleader

ELISA/assay

Mouse BAFF PicoKine ELISA Kit

EK0664

1 x96 well plate

400 USD

Mouse BAFF PicoKine ELISA Kit

EK0664

400 USD

Sandwich ELISA kit for Quantitative Detection of Mouse BAFF

NSO, A127-L309

1 x96 well plate

46.9pg/ml-3000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 139; Standard deviation: 4.9; CV(%): 3.5 Sample 2: n=16; Mean(pg/ml): 312; Standard deviation: 11.5; CV(%): 3.7 Sample 3: n=16; Mean(pg/ml): 1508; Standard deviation: 58.8; CV(%): 3.9

Sample 1: n=24; Mean(pg/ml): 145; Standard deviation: 14.4; CV(%): 9.9 Sample 2: n=24; Mean(pg/ml): 354; Standard deviation: 33.6; CV(%): 9.5 Sample 3: n=24; Mean(pg/ml): 1397; Standard deviation: 124.3; CV(%): 8.9

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse BAFF in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse BAFF antibody 1 ST0000-10 Lyophilized recombinant mouse BAFF standard 10ng/tubex2 AR1107 Biotinylated anti- mouse BAFF antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

B-cell activating factor(BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers(i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF(A127-L309) with molecular weight 23.2KDa.

TNFSF13B

Tumor necrosis factor ligand superfamily member 13B

tumor necrosis factor (ligand) superfamily, member 13b

B lymphocyte stimulator B-cell-activating factor BAFF BLyS CD257 Dendritic cell-derived TNF-like molecule soluble form TALL-1 TN13B_HUMAN TNF- and APOL-related leukocyte expressed ligand 1 TNFSF13B Tumor necrosis factor ligand superfamily member 13b

Q3KP92