Boster Immunoleader

ELISA/assay

Human BAFF PicoKine ELISA Kit

EK0663

1 x96 well plate

400 USD

Human BAFF PicoKine ELISA Kit

EK0663

400 USD

Sandwich ELISA kit for Quantitative Detection of Human BAFF

NSO, A134-L285

1 x96 well plate

62.5pg/ml-4000pg/ml

<2pg/ml

Sample 1: n=16; Mean(pg/ml): 400; Standard deviation: 27.2; CV(%): 6.8 Sample 2: n=16; Mean(pg/ml): 950; Standard deviation: 71.25; CV(%): 7.5 Sample 3: n=16; Mean(pg/ml): 2108; Standard deviation: 90.6; CV(%): 4.3

Sample 1: n=24; Mean(pg/ml): 425; Standard deviation: 39.1; CV(%): 9.2 Sample 2: n=24; Mean(pg/ml): 1093; Standard deviation: 108.2; CV(%): 9.9 Sample 3: n=24; Mean(pg/ml): 2357; Standard deviation: 228.6; CV(%): 9.7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human BAFF in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human BAFF antibody 1 ST0000-10 Lyophilized recombinant human BAFF standard 10ng/tubex2 AR1107 Biotinylated anti- human BAFF antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system(CNS) lymphomas.1 The TNF superfamily member B cell-activating factor(BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology.2 The B cell activating factor BAFF(BlyS/TALL-1/zTNF4) is a tumor necrosis factor(TNF)-related ligand that promotes B cell survival and binds to three receptors(BCMA, TACI, and the recently described BAFF-R).3 Human BAFF was mapped to chromosome 13q32-34.4 The standard used in this kit is recombinant soluble human BAFF(A134-L295) with the molecular mass of 19.6KDa.

TNFSF13B

Tumor necrosis factor ligand superfamily member 13B

tumor necrosis factor (ligand) superfamily, member 13b

B lymphocyte stimulator B-cell-activating factor BAFF BLyS CD257 Dendritic cell-derived TNF-like molecule soluble form TALL-1 TN13B_HUMAN TNF- and APOL-related leukocyte expressed ligand 1 TNFSF13B Tumor necrosis factor ligand superfamily member 13b

Q9Y275