Boster Immunoleader

ELISA/assay

Human APP PicoKine ELISA Kit

EK0658

1 x96 well plate

400 USD

Human APP PicoKine ELISA Kit

EK0658

400 USD

Sandwich ELISA kit for Quantitative Detection of Human APP

NSO, L18-L688

1 x96 well plate

312pg/ml-20,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 3.37; Standard deviation: 0.162; CV(%): 4.8 Sample 2: n=16; Mean(ng/ml): 6.58; Standard deviation: 0.336; CV(%): 5.1 Sample 3: n=16; Mean(ng/ml): 13.29; Standard deviation: 0.704; CV(%): 5.3

Sample 1: n=24; Mean(ng/ml): 3.42; Standard deviation: 0.198; CV(%): 5.8 Sample 2: n=24; Mean(ng/ml): 6.68; Standard deviation: 0.414; CV(%): 6.2 Sample 3: n=24; Mean(ng/ml): 14.21; Standard deviation: 0.895; CV(%): 6.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human APP in cell culture supernates,serum and plasma(heprain, EDTA)

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human APP antibody 1 ST0000-20 Lyophilized recombinant human APP standard 20ng/tubex2 AR1107 Biotinylated anti- human APP antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Amyloid precursor protein(APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates beta amyloid(Abeta), a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients. APP undergoes posttranslational proteolytic processing by alpha-, beta-, and gamma-secretases. Alpha-secretase generates soluble amyloid protein, while beta- and gamma-secretases generate APP components with amyloidogenic features. These 2 processing pathways are mutually exclusive.

APP

Amyloid beta A4 protein

amyloid beta (A4) precursor protein

A4 amyloid protein A4 A4_HUMAN AAA ABETA ABPP AD 1 AD1 AICD-50 AICD-57 AICD-59 AID(50) AID(57) AID(59) Alzheimer disease 1 Alzheimer disease amyloid protein Alzheimer's disease amyloid protein Amyloid beta (A4) precursor protein Amyloid beta A4 protein Amyloid beta A4 protein precursor isoform b Amyloid beta A4 protein precursor isoform b Amyloid beta A4 protein precursor isoform c Amyloid beta A4 protein precursor isoform c Amyloid beta A4 protein precursor isoform a Amyloid beta A4 protein precursor isoform b Amyloid beta A4 protein precursor isoform b Amyloid beta A4 protein precursor isoform c Amyloid beta A4 protein precursor isoform c Amyloid beta protein Amyloid beta-peptide Amyloid intracellular domain 50 Amyloid intracellular domain 57 Amyloid intracellular domain 59 Amyloid of aging and alzheimer disease APP APP I APPI Beta amyloid peptide Beta-APP40 Beta-APP42 C31 Cerebral vascular amyloid peptide CTFgamma CVAP Gamma-CTF(50) Gamma-CTF(57) Gamma-CTF(59) Human mRNA for amyloid A4 precursor of Alzheimer's disease peptidase nexin II PN 2 PN II PN-II PN2 PreA4 Protease nexin II Protease nexin-II S-APP-alpha S-APP-beta

P05067