Boster Immunoleader

ELISA/assay

Human Angiopoietin-2 PicoKine ELISA Kit

EK0657

1 x96 well plate

369 USD

Human Angiopoietin-2 PicoKine ELISA Kit

EK0657

369 USD

Sandwich ELISA kit for Quantitative Detection of Human Angiopoietin-2

NSO, Y19-F496

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 1.08; Standard deviation: 0.049; CV(%): 4.5 Sample 2: n=16; Mean(ng/ml): 3.49; Standard deviation: 0.181; CV(%): 5.2 Sample 3: n=16; Mean(ng/ml): 7.01; Standard deviation: 0.407; CV(%): 5.8

Sample 1: n=24; Mean(ng/ml): 1.05; Standard deviation: 0.071; CV(%): 6.8 Sample 2: n=24; Mean(ng/ml): 3.32; Standard deviation: 0.236; CV(%): 7.1 Sample 3: n=24; Mean(ng/ml): 6.67; Standard deviation: 0.5; CV(%): 7.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Angiopoietin-2 in cell culture supernates, serum, plasma(heparin, EDTA), saliva and human milk.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Angiopoietin-2 antibody 1 ST0000-10 Lyophilized recombinant human Angiopoietin-2 standard 10ng/tubex2 AR1107 Biotinylated anti- human Angiopoietin-2 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml Material Required But Not Provided

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

ANGPT2

Angiopoietin-2

angiopoietin 2

AGPT2 ANG 2 ANG-2 ANG2 Angiopoietin 2a Angiopoietin 2B Angiopoietin-2 Angiopoietin2 ANGP2_HUMAN ANGPT 2 Angpt2 Tie2 ligand

O15123