Boster Immunoleader

ELISA/assay

Human CD105 PicoKine ELISA Kit

EK0644

1 x96 well plate

400 USD

Human CD105 PicoKine ELISA Kit

EK0644

400 USD

Sandwich ELISA kit for Quantitative Detection of Human CD105

CHO, E26-G586

1 x96 well plate

156pg/ml-10,000pg/ml

<15pg/ml

Sample 1: n=16; Mean(ng/ml): 0.62; Standard deviation: 0.022; CV(%): 3.5 Sample 2: n=16; Mean(ng/ml): 1.67; Standard deviation: 0.078; CV(%): 4.7 Sample 3: n=16; Mean(ng/ml): 3.59; Standard deviation: 0.151; CV(%): 4.2

Sample 1: n=24; Mean(ng/ml): 0.71; Standard deviation: 0.051; CV(%): 7.2 Sample 2: n=24; Mean(ng/ml): 1.78; Standard deviation: 0.126; CV(%): 7.1 Sample 3: n=24; Mean(ng/ml): 3.46; Standard deviation: 0.266; CV(%): 7.7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human CD105 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human CD105 antibody 1 ST0000-10 Lyophilized recombinant human CD105 standard 10ng/tubex2 AR1107 Biotinylated anti- human CD105 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta(TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa.

ENG

Endoglin

endoglin

AI528660 AI662476 CD 105 CD105 antigen Cell surface MJ7/18 antigen END Endoglin ENG FLJ41744 HHT1 ORW ORW1 Osler Rendu Weber syndrome 1 OTTMUSP00000012838 OTTMUSP00000012839 RP11 228B15.2 RP23 17P12.4 S endoglin Transmembrane glycoprotein

P17813