Boster Immunoleader

ELISA/assay

Mouse CD40L PicoKine ELISA Kit

EK0574

1 x96 well plate

399 USD

Mouse CD40L PicoKine ELISA Kit

EK0574

399 USD

Sandwich ELISA kit for Quantitative Detection of Mouse CD40L

NSO, E61-L260

1 x96 well plate

62.5pg/ml-4000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 537; Standard deviation: 28.5; CV(%): 5.3 Sample 2: n=16; Mean(pg/ml): 1562; Standard deviation: 71.9; CV(%): 4.6 Sample 3: n=16; Mean(pg/ml): 3025; Standard deviation: 172.4; CV(%): 5.7

Sample 1: n=24; Mean(pg/ml): 562; Standard deviation: 36.53; CV(%): 6.5 Sample 2: n=24; Mean(pg/ml): 1675; Standard deviation: 97.15; CV(%): 5.8 Sample 3: n=24; Mean(pg/ml): 3140; Standard deviation: 207.24; CV(%): 6.6

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse CD40L in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse CD40L antibody 1 ST0000-10 Lyophilized recombinant mouse CD40L standard 10ng/tubex2 AR1107 Biotinylated anti- mouse CD40L antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

CD40 ligand(CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

CD40LG

CD40 ligand

CD40 ligand

CD154 CD40 antigen ligand CD40 ligand CD40-L CD40L_HUMAN CD40LG gp39 HIGM1 IGM IMD3 soluble form T B cell activating molecule T BAM T cell antigen Gp39 T-cell antigen Gp39 TNF related activation protein TNF-related activation protein TNFSF5 TrAP Tumor necrosis factor (ligand) superfamily member 5 Tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome) Tumor necrosis factor ligand superfamily member 5

P27548