Boster Immunoleader

ELISA/assay

Human sCD40L PicoKine ELISA Kit

EK0573

1 x96 well plate

430 USD

Human sCD40L PicoKine ELISA Kit

EK0573

430 USD

Sandwich ELISA kit for Quantitative Detection of Human soluble CD40L

E.coli, E108-L261

1 x96 well plate

62.5pg/ml-4000pg/ml

<15pg/ml

Sample 1: n=16; Mean(ng/ml): 0.44; Standard deviation: 0.026; CV(%): 5.9 Sample 2: n=16; Mean(ng/ml): 1.26; Standard deviation: 0.064; CV(%): 5.1 Sample 3: n=16; Mean(ng/ml): 2.38; Standard deviation: 0.145; CV(%): 6.1

Sample 1: n=24; Mean(ng/ml): 0.42; Standard deviation: 0.029; CV(%): 6.8 Sample 2: n=24; Mean(ng/ml): 1.41; Standard deviation: 0.086; CV(%): 6.1 Sample 3: n=24; Mean(ng/ml): 2.46; Standard deviation: 0.182; CV(%): 7.4

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human CD40L in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human sCD40L antibody 1 ST0000-10 Lyophilized recombinant human sCD40L standard 10ng/tubex2 AR1107 Biotinylated anti- human sCD40L antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

CD40 ligand(CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

CD40LG

CD40 ligand

CD40 ligand

CD154 CD40 antigen ligand CD40 ligand CD40-L CD40L_HUMAN CD40LG gp39 HIGM1 IGM IMD3 soluble form T B cell activating molecule T BAM T cell antigen Gp39 T-cell antigen Gp39 TNF related activation protein TNF-related activation protein TNFSF5 TrAP Tumor necrosis factor (ligand) superfamily member 5 Tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome) Tumor necrosis factor ligand superfamily member 5

P29965