Boster Immunoleader

ELISA/assay

Mouse CD30L PicoKine ELISA Kit

EK0572

1 x96 well plate

380 USD

Mouse CD30L PicoKine ELISA Kit

EK0572

380 USD

Sandwich ELISA kit for Quantitative Detection of Mouse CD30L

NSO, Q68-D239

1 x96 well plate

15.6pg/ml-1000pg/ml

<6pg/ml

Sample 1: n=16; Mean(pg/ml): 92; Standard deviation: 4.51; CV(%): 4.9 Sample 2: n=16; Mean(pg/ml): 349; Standard deviation: 24.08; CV(%): 6.9 Sample 3: n=16; Mean(pg/ml): 661; Standard deviation: 40.98; CV(%): 6.2

Sample 1: n=24; Mean(pg/ml): 105; Standard deviation: 6.93; CV(%): 5.6 Sample 2: n=24; Mean(pg/ml): 357; Standard deviation: 25.35; CV(%): 7.1 Sample 3: n=24; Mean(pg/ml): 711; Standard deviation: 51.90; CV(%): 7.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse CD30L in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse CD30L antibody 1 ST0000-10 Lyophilized recombinant mouse CD30L standard 10ng/tubex2 AR1107 Biotinylated anti- mouse CD30L antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

The human CD30L(CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154(CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that this"reverse signaling" modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45KDa.

TNFSF8

Tumor necrosis factor ligand superfamily member 8

tumor necrosis factor (ligand) superfamily, member 8

CD153 antigen CD30 antigen ligand CD30 L CD30 ligand CD30L CD30LG MGC138144 TNFSF8 Tumor necrosis factor (ligand) superfamily member 8 Tumor necrosis factor ligand superfamily member 8

P32972