Boster Immunoleader

ELISA/assay

Human Cardiotrophin-1 PicoKine ELISA Kit

EK0563

1 x96 well plate

420 USD

info changed 20150507

Human Cardiotrophin-1 PicoKine ELISA Kit

EK0563

420 USD

Sandwich ELISA kit for Quantitative Detection of Human Cardiotrophin-1

E.coli, S2-A201

1 x96 well plate

156pg/ml-10000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 253; Standard deviation: 11.38; CV(%): 4.5 Sample 2: n=16; Mean(pg/ml): 759; Standard deviation: 35.67; CV(%): 4.7 Sample 3: n=16; Mean(pg/ml): 2042; Standard deviation: 98.09; CV(%): 4.9

Sample 1: n=24; Mean(pg/ml): 264; Standard deviation: 12.14; CV(%): 4.6 Sample 2: n=24; Mean(pg/ml): 788; Standard deviation: 37.82; CV(%): 4.8 Sample 3: n=24; Mean(pg/ml): 1946; Standard deviation: 97.30; CV(%): 5.0

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human CT-1 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Cardiotrophin-1 antibody 1 ST0000-10 Lyophilized recombinant human Cardiotrophin-1 standard 10ng/tubex2 AR1107 Biotinylated anti- human Cardiotrophin-1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Cardiotrophin-1(CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor(LIF), ciliary neurotrophic factor(CNTF), oncostatin M(OSM), interleukin-6(IL6), and interleukin-11(IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80% amino acid identity with the 203-amino acid mouse CT-1 sequence; however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.

CTF1

Cardiotrophin-1

cardiotrophin 1

Cardiophin 1 Cardiophin1 Cardiotrophin I Cardiotrophin-1 CardiotrophinI CT 1 CT-1 CT1 CTF 1 Ctf1 CTF1_HUMAN

Q16619