Boster Immunoleader

ELISA/assay

Mouse ACE PicoKine ELISA Kit

EK0558

1 x96 well plate

369 USD

Mouse ACE PicoKine ELISA Kit

EK0558

369 USD

Sandwich ELISA kit for Quantitative Detection of Mouse ACE

NSO, L35-Q1264

1 x96 well plate

93.8pg/ml-6000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 0.64; Standard deviation: 0.029; CV(%): 4.6 Sample 2: n=16; Mean(ng/ml): 1.98; Standard deviation: 0.085; CV(%): 4.3 Sample 3: n=16; Mean(ng/ml): 4.25; Standard deviation: 0.179; CV(%): 4.2

Sample 1: n=24; Mean(ng/ml): 0.85; Standard deviation: 0.062; CV(%): 7.3 Sample 2: n=24; Mean(ng/ml): 2.02; Standard deviation: 0.141; CV(%): 7 Sample 3: n=24; Mean(ng/ml): 5.32; Standard deviation: 0.367; CV(%): 6.9

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse ACE in cell culture supernates, serum and plasma(heparin).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse ACE antibody 1 ST0000-10 Lyophilized recombinant mouse ACE standard 10ng/tubex2 AR1107 Biotinylated anti- mouse ACE antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Angiotensin-converting enzyme(ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system(RAS), which mediates extracellular volume(i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic' and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator.

ACE

Angiotensin-converting enzyme

angiotensin I converting enzyme

Angiotensin converting enzyme CD143 DCP Dcp1 Dipeptidyl carboxypeptidase I Kininase II

P09470