Boster Immunoleader

ELISA/assay

Rat VEGF PicoKine ELISA Kit

EK0540

1 x96 well plate

320 USD

Rat VEGF PicoKine ELISA Kit

EK0540

320 USD

Sandwich ELISA kit for Quantitative Detection of Rat VEGF

NSO,A27-R190

1 x96 well plate

15.6pg/ml-1000pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 120; Standard deviation: 4.44; CV(%): 3.7 Sample 2: n=16; Mean(pg/ml): 305; Standard deviation: 15.86; CV(%): 5.2 Sample 3: n=16; Mean(pg/ml): 621; Standard deviation: 29.81; CV(%): 4.8

Sample 1: n=24; Mean(pg/ml): 236; Standard deviation: 10.62; CV(%): 4.5 Sample 2: n=24; Mean(pg/ml): 289; Standard deviation: 18.21; CV(%): 6.3 Sample 3: n=24; Mean(pg/ml): 632; Standard deviation: 34.76; CV(%): 5.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat VEGF in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat VEGF antibody 1 ST0000-10 Lyophilized recombinant rat VEGF standard 10ng/tubex2 AR1107 Biotinylated anti- rat VEGF antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25KDa single chain as well as a 50KDa dimer.

VEGFA

Vascular endothelial growth factor A

vascular endothelial growth factor A

MGC70609 MVCD1 Vascular endothelial growth factor Vascular endothelial growth factor A Vascular permeability factor VEGF A VEGFA VPF

B5DEK7