product summary
request information :
company name :
Boster Immunoleader
product type :
ELISA/assay
product name :
Mouse VCAM-1 PicoKine ELISA Kit
catalog :
EK0538
quantity :
1 x96 well plate
price :
340 USD
product information
Product Name :
Mouse VCAM-1 PicoKine ELISA Kit
Catalog Number :
EK0538
Price :
340 USD
Description short :
Sandwich ELISA kit for Quantitative Detection of Mouse VCAM-1
immunogen :
NSO,F25 - E698
Pack Size :
1 x96 well plate
Assay Range :
156pg/ml-10,000pg/ml
Sensitivity :
<5pg/ml
Intra-Assay Precision :
Sample 1: n=16; Mean(pg/ml): 230; Standard deviation: 8.74; CV(%): 3.8 Sample 2: n=16; Mean(pg/ml): 2346; Standard deviation: 105.6; CV(%): 4.5 Sample 3: n=16; Mean(pg/ml): 5393; Standard deviation: 280.4; CV(%): 5.2
Inter-Assay Precision :
Sample 1: n=24; Mean(pg/ml): 384; Standard deviation: 17.28; CV(%): 4.5 Sample 2: n=24; Mean(pg/ml): 3017; Standard deviation: 162.9; CV(%): 5.4 Sample 3: n=24; Mean(pg/ml): 6245; Standard deviation: 37; CV(%): 6
Cross Reactivity :
There is no detectable cross-reactivity with other relevant proteins.
Storage :
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Sample Type :
This kit is used for quantitative detection of mouse VCAM-1 in cell culture supernates, serum and plasma(heparin, EDTA).
Reactivity :
Mouse
Kit Components :
Catalog number Description Quantity - 96-well plate precoated with anti- mouse VCAM-1 antibody 1 ST0000-10 Lyophilized recombinant mouse VCAM-1 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse VCAM-1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml
Background :
Vascular cell adhesion molecule 1(VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1(VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9KDa. As a result of glycosylation, the molecular mass of 120KDa is revealed by SDS-PAGE.
Gene Name :
VCAM1
Protein Name :
Vascular cell adhesion protein 1
Gene Full Name :
vascular cell adhesion molecule 1
Synonyms :
CD106 CD106 antigen DKFZp779G2333 INCAM 100 INCAM-100 INCAM100 L1CAM MGC99561 V CAM 1 V-CAM 1 Vascular cell adhesion molecule 1 Vascular cell adhesion protein 1 VCAM 1 VCAM-1 VCAM1 VCAM1_HUMAN
Uniprot IDs :
P29533
company information
Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com
925.485.4527
headquarters: USA
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