Boster Immunoleader

ELISA/assay

Human uPA/PLAU PicoKine ELISA Kit

EK0535

1 x96 well plate

370 USD

Human uPA/PLAU PicoKine ELISA Kit

EK0535

370 USD

Sandwich ELISA kit for Quantitative Detection of Human uPA/PLAU

NSO, M1-L431

1 x96 well plate

62.5pg/ml-4000pg/ml

<5pg/ml

Sample 1: n=16; Mean(pg/ml): 126; Standard deviation: 5.92; CV(%): 4.7 Sample 2: n=16; Mean(pg/ml): 1895; Standard deviation: 64.43; CV(%): 3.4 Sample 3: n=16; Mean(pg/ml): 2619; Standard deviation: 138.8; CV(%): 5.3

Sample 1: n=24; Mean(pg/ml): 250; Standard deviation: 13.5; CV(%): 5.4 Sample 2: n=24; Mean(pg/ml): 1568; Standard deviation: 95.65; CV(%): 6.1 Sample 3: n=24; Mean(pg/ml): 2755; Standard deviation: 187.3; CV(%): 6.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human uPA in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human uPA/PLAU antibody 1 ST0000-10 Lyophilized recombinant human uPA/PLAU standard 10ng/tubex2 AR1107 Biotinylated anti- human uPA/PLAU antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Plasminogen activator,urokinase(PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease(AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell(VSMC), uPA stimulates migration via the uPA receptor(uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase(PI3-K).

PLAU

Urokinase-type plasminogen activator

plasminogen activator, urokinase

ATF BDPLT5 Plasminogen activator urinary PLAU QPD u PA U-plasminogen activator uPA URK UROK_HUMAN Urokinase-type plasminogen activator chain B

P00749