Boster Immunoleader

ELISA/assay

Human TRAIL PicoKine ELISA Kit

EK0532

1 x96 well plate

400 USD

Human TRAIL PicoKine ELISA Kit

EK0532

400 USD

Sandwich ELISA kit for Quantitative Detection of Human TRAIL

NSO,T95-G281

1 x96 well plate

15.6pg/ml-1000pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 102; Standard deviation: 4.59; CV(%): 4.5 Sample 2: n=16; Mean(pg/ml): 368; Standard deviation: 12.88; CV(%): 3.5 Sample 3: n=16; Mean(pg/ml): 637; Standard deviation: 39.5; CV(%): 6.2

Sample 1: n=24; Mean(pg/ml): 167; Standard deviation: 13.36; CV(%): 8 Sample 2: n=24; Mean(pg/ml): 326; Standard deviation: 24.45; CV(%): 7.5 Sample 3: n=24; Mean(pg/ml): 624; Standard deviation: 48.67; CV(%): 7.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human TRAIL in cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) and saliva.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human TRAIL antibody 1 ST0000-10 Lyophilized recombinant human TRAIL standard 10ng/tubex2 AR1107 Biotinylated anti- human TRAIL antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

In the field of cell biology, TNF-related apoptosis-inducing ligand(TRAIL), is a protein which functions as a ligand which induces the process of cell death called apoptosis. TRAIL has also been designated CD253(cluster of differentiation 253).Tumor necrosis factor(TNF) family cytokines function as prominent mediators of immune regulation and the inflammatory response. Most TNF family cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallel family of receptors. TRAIL binds to the death receptors DR4(TRAIL-RI) and DR5(TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain(DcR1) or contain a truncated death domain(DcR2). The standard product used in this kit is recombinant human TRAIL with the molecular mass of 21KDa. It is expressed from the amino acids ectoderm T95--G28.

TNFSF10

Tumor necrosis factor ligand superfamily member 10

tumor necrosis factor (ligand) superfamily, member 10

Apo 2 ligand APO2L CD253 Protein TRAIL TL2 TNF related apoptosis inducing ligand Tumor necrosis factor ligand superfamily member 10

P50591