Boster Immunoleader

ELISA/assay

Human TNFsR I PicoKine ELISA Kit

EK0528

1 x96 well plate

400 USD

Human TNFsR I PicoKine ELISA Kit

EK0528

400 USD

Sandwich ELISA kit for Quantitative Detection of Human TNFsR I

NSO,L30-T211

1 x96 well plate

7.8pg/ml-500pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 74; Standard deviation: 2.52; CV(%): 3.4 Sample 2: n=16; Mean(pg/ml): 133; Standard deviation: 6.52; CV(%): 4.9 Sample 3: n=16; Mean(pg/ml): 261; Standard deviation: 14.9; CV(%): 5.7

Sample 1: n=24; Mean(pg/ml): 85; Standard deviation: 3.91; CV(%): 4.6 Sample 2: n=24; Mean(pg/ml): 156; Standard deviation: 7.96; CV(%): 5.1 Sample 3: n=24; Mean(pg/ml): 278; Standard deviation: 18.07; CV(%): 6.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human TNF s RI in cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human TNFsR I antibody 1 ST0000-10 Lyophilized recombinant human TNFsR I standard 10ng/tubex2 AR1107 Biotinylated anti- human TNFsR I antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

TNFsRI(also known as Solube tumor necrosis factor receptor 1) is a cytokine receptor that binds tumor necrosis factors(TNF). In contrast, the p60(TNFRSF1A) and p80(TNFRSF1B) TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain(PLAD), in the absence of ligand. Deletion of the PLAD results in monomeric presentation of p60 or p80. Flow cytometric analysis showed that efficient TNFA binding depends on receptor self-assembly. They also found that other members of the TNF receptor superfamily, including the extracellular domains of TRAIL(TNFRSF10A), CD40, and FAS(TNFRSF6), all self-associate but do not interact with heterologous receptors. By Southern blot analysis of human/Chinese hamster somatic cell hybrid DNA, the TNFR1 gene was mapped to 12pter-cen. And by nonradioactive in situ hybridization that the type 1 receptor(the p55 TNF receptor) is encoded by a gene located on chromosome 12p13.2.

TNFRSF1A

Tumor necrosis factor receptor superfamily member 1A

tumor necrosis factor receptor superfamily, member 1A

CD120a FPF p55 p55 R p60 p65 TBP1 TBPI TNF R TNF R I TNF R55 TNF-R1 TNF-RI TNFAR TNFR I TNFR1 TNFR55 TNFR60 TNFRI TNFRSF1A TNR1A_HUMAN Tumor necrosis factor alpha receptor Tumor necrosis factor binding protein 1 Tumor necrosis factor receptor 1 Tumor necrosis factor receptor superfamily member 1A Tumor necrosis factor receptor superfamily member 1A, membrane form Tumor necrosis factor receptor type I Tumor necrosis factor-binding protein 1

P19438