Boster Immunoleader

ELISA/assay

Rat TNF alpha PicoKine ELISA Kit

EK0526

1 x96 well plate

320 USD

Rat TNF alpha PicoKine ELISA Kit

EK0526

320 USD

Sandwich ELISA kit for Quantitative Detection of Rat TNF alpha

E.coli,L80-L235

1 x96 well plate

15.6pg/ml-1000pg/ml(cellculturesupernates),7.8pg/ml-500pg/ml(serum,plasma)

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 90; Standard deviation: 5.3; CV(%): 5.3 Sample 2: n=16; Mean(pg/ml): 245; Standard deviation: 3.1; CV(%): 3.1 Sample 3: n=16; Mean(pg/ml): 612; Standard deviation: 3.4; CV(%): 3.4

Sample 1: n=24; Mean(pg/ml): 103; Standard deviation: 6.9; CV(%): 6.7 Sample 2: n=24; Mean(pg/ml): 261; Standard deviation: 15.4; CV(%): 5.9 Sample 3: n=24; Mean(pg/ml): 684; Standard deviation: 44.5; CV(%): 6.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat TNF alpha in cell culture supernates, serum and plasma(heparin, EDTA).

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat TNF alpha antibody 1 ST0000-10 Lyophilized recombinant rat TNF alpha standard 10ng/tubex2 AR1107 Biotinylated anti- rat TNF alpha antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFalpha, consisting of 157 amino acids with the molecular mass of 17KDa.

TNF

Tumor necrosis factor

tumor necrosis factor

APC1 protein Cachectin DIF soluble form Tnf TNF a TNF alpha TNF macrophage derived TNF monocyte derived TNF superfamily member 2 TNF-a TNF-alpha TNFA_HUMAN TNFSF2 Tumor necrosis factor Tumor necrosis factor alpha Tumor necrosis factor ligand superfamily member 2

P16599