Boster Immunoleader

ELISA/assay

Human TNF alpha PicoKine ELISA Kit

EK0525

1 x96 well plate

250 USD

Human TNF alpha PicoKine ELISA Kit

EK0525

250 USD

Sandwich ELISA kit for Quantitative Detection of Human TNF alpha

E.coli,V77-L233

1 x96 well plate

15.6pg/ml-1000pg/ml(cellculturesupernates),7.8pg/ml-500pg/ml(serum,plasma)

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 93; Standard deviation: 5.1; CV(%): 5.5 Sample 2: n=16; Mean(pg/ml): 327; Standard deviation: 15.4; CV(%): 4.7 Sample 3: n=16; Mean(pg/ml): 608; Standard deviation: 31; CV(%): 5.1

Sample 1: n=24; Mean(pg/ml): 102; Standard deviation: 7.65; CV(%): 7.5 Sample 2: n=24; Mean(pg/ml): 319; Standard deviation: 15.3; CV(%): 4.8 Sample 3: n=24; Mean(pg/ml): 613; Standard deviation: 35; CV(%): 5.7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human TNF alpha in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human TNF alpha antibody 1 ST0000-10 Lyophilized recombinant human TNF alpha standard 10ng/tubex2 AR1107 Biotinylated anti- human TNF alpha antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

TNF

Tumor necrosis factor

tumor necrosis factor

APC1 protein Cachectin DIF soluble form Tnf TNF a TNF alpha TNF macrophage derived TNF monocyte derived TNF superfamily member 2 TNF-a TNF-alpha TNFA_HUMAN TNFSF2 Tumor necrosis factor Tumor necrosis factor alpha Tumor necrosis factor ligand superfamily member 2

P01375