Boster Immunoleader

ELISA/assay

Human TIE2 PicoKine ELISA Kit

EK0519

1 x96 well plate

380 USD

Human TIE2 PicoKine ELISA Kit

EK0519

380 USD

Sandwich ELISA kit for Quantitative Detection of Human TIE2

NSO, A23-K745

1 x96 well plate

156pg/ml-10,000pg/ml

<5pg/ml

Sample 1: n=16; Mean(ng/ml): 0.87; Standard deviation: 0.048; CV(%): 5.5 Sample 2: n=16; Mean(ng/ml): 2.75; Standard deviation: 0.132; CV(%): 4.8 Sample 3: n=16; Mean(ng/ml): 6.3; Standard deviation: 0.334; CV(%): 5.3

Sample 1: n=24; Mean(ng/ml): 1.01; Standard deviation: 0.088; CV(%): 8.7 Sample 2: n=24; Mean(ng/ml): 2.88; Standard deviation: 0.19; CV(%): 6.6 Sample 3: n=24; Mean(ng/ml): 7.2; Standard deviation: 0.422; CV(%): 5.7

There is cross-reactivates with human TIE1< 0.1%.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human TIE2 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human TIE2 antibody 1 ST0000-10 Lyophilized recombinant human TIE2 standard 10ng/tubex2 AR1107 Biotinylated anti- human TIE2 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Tyrosine kinase with Ig and EGF homology domain 2(Tie-2), also called TEK tyrosine kinase, endothelial(TEK). Tie-2 and tie-1 are expressed in early embryonic vascular system and in maternal decidual vascular endothelial cells, where the vasculature undergoes an active angiogenesis. Tie-2, but not tie-1, expression was also detected in extraembryonic mesoderm of the amnion. Angiogenesis is coordinated with follicular cell growth in goitrogenesis. The angiopoietins, Ang-1 and Ang-2, are angiogenic growth factors acting through Tie-2. Tie-2 and Ang-1 are expressed in thyroid epithelial and endothelial cells, and Tie-2 is regulated by TSH and cAMP in follicular cells. And Tie-2 expression is increased in goiter in both humans and rats, consistent with a role in goitrogenesis. Tie2/Ang-1 signaling pathway plays a critical role in the maintenance of HSCs in a quiescent state in the BM niche. And the Tie-2 signaling pathway is also critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

TEK

Angiopoietin-1 receptor

TEK tyrosine kinase, endothelial

Angiopoietin-1 receptor CD202b CD202b antigen EC 2.7.10.1 hTIE2 p140 TEK soluble TIE2 variant 1 soluble TIE2 variant 2 Tek TEK tyrosine kinase, endothelial TIE 2 TIE2_HUMAN Tunica interna endothelial cell kinase Tyrosine-protein kinase receptor TEK Tyrosine-protein kinase receptor TIE 2 Tyrosine-protein kinase receptor TIE-2 venous malformations, multiple cutaneous and mucosal VMCM VMCM1

Q02763