Boster Immunoleader

ELISA/assay

Mouse TPO PicoKine ELISA Kit

EK0517

1 x96 well plate

340 USD

Mouse TPO PicoKine ELISA Kit

EK0517

340 USD

Sandwich ELISA kit for Quantitative Detection of Mouse TPO

NSO, S22-T356

1 x96 well plate

31.2pg/ml-2000pg/ml

<5pg/ml

Sample 1: n=16; Mean(pg/ml): 296; Standard deviation: 11.84; CV(%): 4 Sample 2: n=16; Mean(pg/ml): 611; Standard deviation: 33; CV(%): 5.4 Sample 3: n=16; Mean(pg/ml): 1265; Standard deviation: 82.23; CV(%): 6.5

Sample 1: n=24; Mean(pg/ml): 321; Standard deviation: 16.7; CV(%): 5.2 Sample 2: n=24; Mean(pg/ml): 746; Standard deviation: 47; CV(%): 6.3 Sample 3: n=24; Mean(pg/ml): 1464; Standard deviation: 105.4; CV(%): 7.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse TPO in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse TPO antibody 1 ST0000-10 Lyophilized recombinant mouse TPO standard 10ng/tubex2 AR1107 Biotinylated anti- mouse TPO antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Thrombopoietin(TPO) is also called megakaryocyte growth and development factor(MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.

TPO

Thrombopoietin

thyroid peroxidase

C mpl ligand Megakaryocyte colony stimulating factor Megakaryocyte growth and development factor Megakaryocyte stimulating factor MGC163194 MGDF MKCSF ML MPL ligand MPLLG Myeloproliferative leukemia virus oncogene ligand OTTHUMP00000210504 THPO TPO

P40226