Boster Immunoleader

ELISA/assay

Rat TGF beta 1 PicoKine ELISA Kit

EK0514

1 x96 well plate

320 USD

Rat TGF beta 1 PicoKine ELISA Kit

EK0514

320 USD

Sandwich ELISA kit for Quantitative Detection of Rat TGF beta 1

CHO, A279-S390

1 x96 well plate

15.6pg/ml-1000pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 122; Standard deviation: 4.15; CV(%): 3.4 Sample 2: n=16; Mean(pg/ml): 253; Standard deviation: 11.4; CV(%): 4.5 Sample 3: n=16; Mean(pg/ml): 506; Standard deviation: 25.81; CV(%): 5.1

Sample 1: n=24; Mean(pg/ml): 140; Standard deviation: 6.72; CV(%): 4.8 Sample 2: n=24; Mean(pg/ml): 256; Standard deviation: 14.08; CV(%): 5.5 Sample 3: n=24; Mean(pg/ml): 613; Standard deviation: 37.39; CV(%): 6.1

There is cross-reactivates with TGFbeta2, TGFbeta3, TGFbeta5<1%.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of activated rat TGF beta 1 in cell culture supernates, serum, plasma(EDTA) and urine.

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat TGF beta 1 antibody 1 ST0000-10 Lyophilized recombinant rat TGF beta 1 standard 10ng/tubex2 AR1107 Biotinylated anti- rat TGF beta 1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Transforming growth factor-beta1(TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects. TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGFbeta1 is also an important mediator of bone remodeling. TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.

TGFB1

Transforming growth factor beta-1

transforming growth factor, beta 1

Camurati-Engelmann disease CED DPD1 LAP Latency-associated peptide TGF beta 1 protein TGF-beta-1 TGFB tgfb1 TGFB1_HUMAN TGFbeta Transforming growth factor beta 1

P17246