Boster Immunoleader

ELISA/assay

Human TGF alpha PicoKine ELISA Kit

EK0511

1 x96 well plate

380 USD

Human TGF alpha PicoKine ELISA Kit

EK0511

380 USD

Sandwich ELISA kit for Quantitative Detection of Human TGF alpha

E.coli,V40-A89

1 x96 well plate

15.6pg/ml-1000pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 118; Standard deviation: 5.43; CV(%): 4.6 Sample 2: n=16; Mean(pg/ml): 225; Standard deviation: 12.83; CV(%): 5.7 Sample 3: n=16; Mean(pg/ml): 516; Standard deviation: 16.5; CV(%): 3.2

Sample 1: n=24; Mean(pg/ml): 126; Standard deviation: 8.95; CV(%): 7.1 Sample 2: n=24; Mean(pg/ml): 238; Standard deviation: 14.28; CV(%): 6 Sample 3: n=24; Mean(pg/ml): 509; Standard deviation: 27; CV(%): 5.3

There is cross-reactivates with TGF beta 2, TGF beta 3, TGF beta 5<1%.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human TGF alpha in cell culture supernates, serum, plasma(heparin, EDTA) and human milk.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human TGF alpha antibody 1 ST0000-10 Lyophilized recombinant human TGF alpha standard 10ng/tubex2 AR1107 Biotinylated anti- human TGF alpha antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Transforming growth factor alpha(TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to human chromosome 2 close to the breakpoint of the t(2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

TGFA

Protransforming growth factor alpha

transforming growth factor, alpha

EGF like TGF ETGF OTTHUMP00000203300 OTTHUMP00000203301 OTTHUMP00000203345 OTTHUMP00000203352 Protransforming growth factor alpha RATTGFAA TFGA TGF A TGF type 1 TGFA Transforming growth factor alpha Transforming growth factor alpha precursor Wa1 Waved 1

P01135