Boster Immunoleader

ELISA/assay

Rat SCF PicoKine ELISA Kit

EK0508

1 x96 well plate

369 USD

Rat SCF PicoKine ELISA Kit

EK0508

369 USD

Sandwich ELISA kit for Quantitative Detection of Rat SCF

E.coli, Q26-A190

1 x96 well plate

31.2pg/ml-2000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 115; Standard deviation: 5.17; CV(%): 4.5 Sample 2: n=16; Mean(pg/ml): 450; Standard deviation: 25.65; CV(%): 5.7 Sample 3: n=16; Mean(pg/ml): 1558; Standard deviation: 102.82; CV(%): 6.6

Sample 1: n=24; Mean(pg/ml): 120; Standard deviation: 6.96; CV(%): 5.8 Sample 2: n=24; Mean(pg/ml): 400; Standard deviation: 24.4; CV(%): 6.1 Sample 3: n=24; Mean(pg/ml): 1889; Standard deviation: 119; CV(%): 6.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat SCF in cell culture supernates, serum and plasma(EDTA).

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat SCF antibody 1 ST0000-10 Lyophilized recombinant rat SCF standard 10ng/tube×2 AR1107 Biotinylated anti- rat SCF antibody 130μl(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.

KITLG

Kit ligand

Stem Cell Factor (SCF)

C kit ligand Ckit ligand Kit ligand Kitl KITLG KL 1 KL1 Mast cell growth factor MGF SF SHEP7 Steel factor Stem cell factor Stem cell factor precursor

P21581