Boster Immunoleader

ELISA/assay

Mouse P-Selectin PicoKine ELISA Kit

EK0506

1 x96 well plate

320 USD

Mouse P-Selectin PicoKine ELISA Kit

EK0506

320 USD

Sandwich ELISA kit for Quantitative Detection of Mouse P-Selectin

NSO, W42-A709

1 x96 well plate

62.5pg/ml-4000pg/ml

<5pg/ml

Sample 1: n=16; Mean(pg/ml): 325; Standard deviation: 14; CV(%): 4.3 Sample 2: n=16; Mean(pg/ml): 874; Standard deviation: 43.7; CV(%): 5 Sample 3: n=16; Mean(pg/ml): 1886; Standard deviation: 71.7; CV(%): 3.8

Sample 1: n=24; Mean(pg/ml): 417; Standard deviation: 27.11; CV(%): 6.5 Sample 2: n=24; Mean(pg/ml): 1029; Standard deviation: 63.8; CV(%): 6.2 Sample 3: n=24; Mean(pg/ml): 2020; Standard deviation: 94.94; CV(%): 4.7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse P-Selectin in cell culture supernates, serum and plasma(citrate).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse P-Selectin antibody 1 ST0000-20 Lyophilized recombinant mouse P-Selectin standard 20ng/tubex2 AR1107 Biotinylated anti- mouse P-Selectin antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.

SELP

P-selectin

selectin P (granule membrane protein 140kDa, antigen CD62)

Antigen CD62 CD62 CD62 antigen like family member P CD62 antigen-like family member P CD62P FLJ45155 GMP 140 GMP-140 GMP140 Granule membrane protein 140 Granule membrane protein 140kDa Granulocyte membrane protein GRMP LECAM3 Leukocyte endothelial cell adhesion molecule 3 Leukocyte-endothelial cell adhesion molecule 3 LYAM3_HUMAN P selectin P-selectin PADGEM Platelet activation dependent granule external membrane protein Platelet activation dependent granule-external membrane protein Platelet alpha granule membrane protein PSEL Selectin P Selectin P (granule membrane protein 140kDa antigen CD62) SELP

Q01102