Boster Immunoleader

ELISA/assay

Mouse sE-Selectin PicoKine ELISA Kit

EK0502

1 x96 well plate

369 USD

Mouse sE-Selectin PicoKine ELISA Kit

EK0502

369 USD

Sandwich ELISA kit for Quantitative Detection of Mouse sE-Selectin

NSO, W22-P557

1 x96 well plate

62.5pg/ml-4000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 797; Standard deviation: 30.29; CV(%): 3.8 Sample 2: n=16; Mean(pg/ml): 1320; Standard deviation: 85.8; CV(%): 6.5 Sample 3: n=16; Mean(pg/ml): 2756; Standard deviation: 140.56; CV(%): 5.1

Sample 1: n=24; Mean(pg/ml): 973; Standard deviation: 44.76; CV(%): 4.6 Sample 2: n=24; Mean(pg/ml): 2185; Standard deviation: 157.3; CV(%): 7.2 Sample 3: n=24; Mean(pg/ml): 3360; Standard deviation: 218.4; CV(%): 6.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse soluble E-Selectin in cell culture supernates, serum and plasma(heparin, citrate).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse sE-Selectin antibody 1 ST0000-20 Lyophilized recombinant mouse sE-Selectin standard 20ng/tubex2 AR1107 Biotinylated anti- mouse sE-Selectin antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM). A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.

SELE

E-selectin

selectin E

CD62 antigen-like family member E CD62E CD62E antigen E selectin E-selectin ELAM ELAM-1 ELAM1 Endothelial leukocyte adhesion molecule 1 ESEL LECAM2 Leukocyte endothelial cell adhesion molecule 2 Leukocyte-endothelial cell adhesion molecule 2 LYAM2_HUMAN sele

Q00690