Rat PDGF-AB PicoKine ELISA Kit | Boster Immunoleader EK0485 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

ELISA/assay

product name :

Rat PDGF-AB PicoKine ELISA Kit

catalog :

EK0485

quantity :

1 x96 well plate

price :

320 USD

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Rat PDGF-AB PicoKine ELISA Kit

Catalog Number :

EK0485

Price :

320 USD

Description short :

Sandwich ELISA kit for Quantitative Detection of Rat PDGF-AB

immunogen :

E.coli, (R86-K204)+(S74-T182)

Pack Size :

1 x96 well plate

Assay Range :

31.2pg/ml-2000pg/ml

Sensitivity :

<3pg/ml

Intra-Assay Precision :

Sample 1: n=16; Mean(pg/ml): 246; Standard deviation: 14.27; CV(%): 5.8 Sample 2: n=16; Mean(pg/ml): 632; Standard deviation: 34.76; CV(%): 5.5 Sample 3: n=16; Mean(pg/ml): 1068; Standard deviation: 50.2; CV(%): 4.7

Inter-Assay Precision :

Sample 1: n=24; Mean(pg/ml): 275; Standard deviation: 20.35; CV(%): 7.4 Sample 2: n=24; Mean(pg/ml): 723; Standard deviation: 45.55; CV(%): 6.3 Sample 3: n=24; Mean(pg/ml): 1249; Standard deviation: 73.7; CV(%): 5.9

Cross Reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Storage :

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Sample Type :

This kit is used for quantitative detection of rat PDGF-AB in cell culture supernates, serum and plasma(heparin, EDTA).

Reactivity :

Rat

Kit Components :

Catalog number Description Quantity - 96-well plate precoated with anti- rat PDGF-AB antibody 1 ST0000-10 Lyophilized recombinant rat PDGF-AB standard 10ng/tubex2 AR1107 Biotinylated anti- rat PDGF-AB antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Background :

The platelet-derived growth factor(PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly(96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus(SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog(PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant rat PDGF-AB(A chain Ser87-Arg196; B chain Ser74-Thr182) with the molecular mass of 24.8KDa.

Gene Name :

PDGFB,PDGFA

Protein Name :

Platelet-derived growth factor subunit B,Platelet-derived growth factor subunit A

Gene Full Name :

platelet-derived growth factor beta polypeptide,platelet-derived growth factor alpha polypeptide

Synonyms :

PDGF A chain PDGF B chain PDGF1 PDGF2 PDGFA PDGFB Platelet derived growth factor AB heterodimer

Uniprot IDs :

G3V882, P28576

more info or order :

Boster Immunoleader product webpage