Boster Immunoleader

ELISA/assay

Human PDGF-AB PicoKine ELISA Kit

EK0484

1 x96 well plate

300 USD

Human PDGF-AB PicoKine ELISA Kit

EK0484

300 USD

Sandwich ELISA kit for Quantitative Detection of Human PDGF-AB

E.coli, (S87-T211)+(S82-T190)

1 x96 well plate

31.2pg/ml-2000pg/ml

<2pg/ml

Sample 1: n=16; Mean(pg/ml): 237; Standard deviation: 11.6; CV(%): 4.9 Sample 2: n=16; Mean(pg/ml): 532; Standard deviation: 27.7; CV(%): 5.2 Sample 3: n=16; Mean(pg/ml): 974; Standard deviation: 62.4; CV(%): 6.3

Sample 1: n=24; Mean(pg/ml): 254; Standard deviation: 13.97; CV(%): 5.5 Sample 2: n=24; Mean(pg/ml): 683; Standard deviation: 45.8; CV(%): 6.7 Sample 3: n=24; Mean(pg/ml): 1205; Standard deviation: 95.2; CV(%): 7.9

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human PDGF-AB in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human PDGF-AB antibody 1 ST0000-10 Lyophilized recombinant human PDGF-AB standard 10ng/tubex2 AR1107 Biotinylated anti- human PDGF-AB antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

The platelet-derived growth factor(PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly(96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus(SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog(PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant human PDGF-AB with the molecular mass of 27KDa.

PDGFB,PDGFA

Platelet-derived growth factor subunit B,Platelet-derived growth factor subunit A

platelet-derived growth factor beta polypeptide,platelet-derived growth factor alpha polypeptide

PDGF A chain PDGF B chain PDGF1 PDGF2 PDGFA PDGFB Platelet derived growth factor AB heterodimer

P01127, P04085