Boster Immunoleader

ELISA/assay

Human OPN PicoKine ELISA Kit

EK0482

1 x96 well plate

350 USD

Human OPN PicoKine ELISA Kit

EK0482

350 USD

Sandwich ELISA kit for Quantitative Detection of Human OPN

NSO, I17-N300

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 2.5; Standard deviation: 0.105; CV(%): 4.2 Sample 2: n=16; Mean(ng/ml): 4.3; Standard deviation: 0.146; CV(%): 3.4 Sample 3: n=16; Mean(ng/ml): 6.1; Standard deviation: 0.317; CV(%): 5.2

Sample 1: n=24; Mean(ng/ml): 2.6; Standard deviation: 0.161; CV(%): 6.2 Sample 2: n=24; Mean(ng/ml): 3.9; Standard deviation: 0.226; CV(%): 5.8 Sample 3: n=24; Mean(ng/ml): 6.3; Standard deviation: 0.353; CV(%): 5.6

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human OPN in cell culture supernates, serum, plasma(heparin, EDTA), human milk and urine.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human OPN antibody 1 ST0000-10 Lyophilized recombinant human OPN standard 10ng/tubex2 AR1107 Biotinylated anti- human OPN antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Osteopontin(OPN) also called urinary stone protein, secreted phosphoprotein 1(SPP1), bone sialoprotein, and early T lymphocyte activation 1(ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein(34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.

SPP1

Osteopontin

secreted phosphoprotein 1

BNSP Bone sialoprotein 1 Bone sialoprotein I BSPI Early T lymphocyte activation 1 ETA 1 MGC110940 Nephropontin OPN Osteopontin OSTP_HUMAN Secreted phosphoprotein 1 Secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1) Secreted phosphoprotein 1 (osteopontin bone sialoprotein) SPP-1 SPP1 Urinary stone protein Uropontin

P10451