Boster Immunoleader

ELISA/assay

Rat NGF/NGF beta PicoKine ELISA Kit

EK0471

1 x96 well plate

300 USD

Rat NGF/NGF beta PicoKine ELISA Kit

EK0471

300 USD

Sandwich ELISA kit for Quantitative Detection of Rat NGF/NGF beta

NSO, S122-G241

1 x96 well plate

15.6pg/ml-1000pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 84; Standard deviation: 4.62; CV(%): 5.5 Sample 2: n=16; Mean(pg/ml): 366; Standard deviation: 16.84; CV(%): 4.6 Sample 3: n=16; Mean(pg/ml): 628; Standard deviation: 37.68; CV(%): 6

Sample 1: n=24; Mean(pg/ml): 117; Standard deviation: 6.9; CV(%): 5.9 Sample 2: n=24; Mean(pg/ml): 396; Standard deviation: 20.2; CV(%): 5.1 Sample 3: n=24; Mean(pg/ml): 735; Standard deviation: 52.92; CV(%): 7.2

There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat NGF in cell culture supernates and serum.

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat NGF/NGF beta antibody 1 ST0000-10 Lyophilized recombinant rat NGF/NGF beta standard 10ng/tubex2 AR1107 Biotinylated anti- rat NGF/NGF beta antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.

NGF

Beta-nerve growth factor

nerve growth factor (beta polypeptide)

Beta nerve growth factor Beta NGF HSAN5 MGC161426 MGC161428 Nerve growth factor (beta polypeptide) Nerve growth factor beta polypeptide Nerve growth factor beta subunit NGFB NID67

P25427