Boster Immunoleader

ELISA/assay

Human MMP13 PicoKine ELISA Kit

EK0468

1 x96 well plate

400 USD

Human MMP13 PicoKine ELISA Kit

EK0468

400 USD

Sandwich ELISA kit for Quantitative Detection of Human MMP13

NSO, L20-C471

1 x96 well plate

78pg/ml-5000pg/ml

<5pg/ml

Sample 1: n=16; Mean(pg/ml): 471; Standard deviation: 19.8; CV(%): 4.2 Sample 2: n=16; Mean(pg/ml): 1623; Standard deviation: 86.02; CV(%): 5.3 Sample 3: n=16; Mean(pg/ml): 3245; Standard deviation: 194.7; CV(%): 6

Sample 1: n=24; Mean(pg/ml): 538; Standard deviation: 29.1; CV(%): 5.4 Sample 2: n=24; Mean(pg/ml): 1776; Standard deviation: 104.8; CV(%): 5.9 Sample 3: n=24; Mean(pg/ml): 3685; Standard deviation: 265.32; CV(%): 7.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human MMP13 in cell culture supernates, serum and plasma(heparin).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human MMP13 antibody 1 ST0000-10 Lyophilized recombinant human MMP13 standard 10ng/tubex2 AR1107 Biotinylated anti- human MMP13 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

MMP13

Collagenase 3

matrix metallopeptidase 13 (collagenase 3)

CLG 3 CLG3 Collagenase 3 Collagenase3 MANDP1 Matrix metalloproteinase 13 Matrix metalloproteinase-13 MMP 13 MMP-13 Mmp13 MMP13_HUMAN UMRCASE

A8K846