Boster Immunoleader

ELISA/assay

Human MMP-9 PicoKine ELISA Kit

EK0465

1 x96 well plate

430 USD

Human MMP-9 PicoKine ELISA Kit

EK0465

430 USD

Sandwich ELISA kit for Quantitative Detection of Human MMP-9

NSO, A20-D707

1 x96 well plate

156pg/ml-10,000pg/ml

<5pg/ml

Sample 1: n=16; Mean(ng/ml): 1.35; Standard deviation: 0.042; CV(%): 3.1 Sample 2: n=16; Mean(ng/ml): 3.8; Standard deviation: 0.16; CV(%): 4.2 Sample 3: n=16; Mean(ng/ml): 6.9; Standard deviation: 0.24; CV(%): 3.5

Sample 1: n=24; Mean(ng/ml): 1.7; Standard deviation: 0.095; CV(%): 5.6 Sample 2: n=24; Mean(ng/ml): 3.9; Standard deviation: 0.308; CV(%): 7.9 Sample 3: n=24; Mean(ng/ml): 7.1; Standard deviation: 0.511; CV(%): 7.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human MMP-9 in cell culture supernates, serum, plasma(heparin), saliva and urine.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human MMP-9 antibody 1 ST0000-10 Lyophilized recombinant human MMP-9 standard 10ng/tubex2 AR1107 Biotinylated anti- human MMP-9 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9(MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases(MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.

MMP9

Matrix metalloproteinase-9

matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

82 kDa matrix metalloproteinase-9 92 kDa gelatinase 92 kDa type IV collagenase CLG4B Collagenase Type 4 beta Collagenase type IV 92 KD EC 3.4.24.35 Gelatinase 92 KD Gelatinase B Gelatinase beta GelatinaseB GELB Macrophage gelatinase MANDP2 Matrix metallopeptidase 9 (gelatinase B 92kDa gelatinase 92kDa type IV collangenase) Matrix metalloproteinase 9 MMP 9 MMP-9 MMP9 MMP9_HUMAN Type V collagenase

P14780