Boster Immunoleader

ELISA/assay

Human MMP-8 PicoKine ELISA Kit

EK0464

1 x96 well plate

430 USD

Human MMP-8 PicoKine ELISA Kit

EK0464

430 USD

Sandwich ELISA kit for Quantitative Detection of Human MMP-8

NSO, F21-G467

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 1275; Standard deviation: 56.1; CV(%): 4.4 Sample 2: n=16; Mean(pg/ml): 3563; Standard deviation: 185.3; CV(%): 5.2 Sample 3: n=16; Mean(pg/ml): 6822; Standard deviation: 395.7; CV(%): 5.8

Sample 1: n=24; Mean(pg/ml): 1370; Standard deviation: 69.87; CV(%): 5.1 Sample 2: n=24; Mean(pg/ml): 3728; Standard deviation: 242.32; CV(%): 6.5 Sample 3: n=24; Mean(pg/ml): 7142; Standard deviation: 521.4; CV(%): 7.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human MMP-8 in cell culture supernates, serum, plasma(heparin) and saliva.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human MMP-8 antibody 1 ST0000-10 Lyophilized human MMP-8 standard 10ng/tubex2 AR1107 Biotinylated anti- human MMP-8 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Matrix metalloproteinase 8(MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase(HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

MMP8

Neutrophil collagenase

matrix metallopeptidase 8 (neutrophil collagenase)

BB138268 CLG 1 CLG1 Collagenase 1 Collagenase 1 neutrophil HNC Matrix metallopeptidase 8 Matrix metalloproteinase 8 (neutrophil collagenase) Matrix metalloproteinase-8 MMP 8 MMP-8 Mmp8 MMP8_HUMAN Neutrophil collagenase PMNL CL PMNL collagenase PMNL-CL

P22894