Boster Immunoleader

ELISA/assay

Mouse MMP-3 PicoKine ELISA Kit

EK0462

1 x96 well plate

380 USD

Mouse MMP-3 PicoKine ELISA Kit

EK0462

380 USD

Sandwich ELISA kit for Quantitative Detection of Mouse MMP-3

NSO, Y18-C477

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 1385; Standard deviation: 76.2; CV(%): 5.5 Sample 2: n=16; Mean(pg/ml): 3758; Standard deviation: 233; CV(%): 6.2 Sample 3: n=16; Mean(pg/ml): 6772; Standard deviation: 291.2; CV(%): 4.3

Sample 1: n=24; Mean(pg/ml): 1479; Standard deviation: 99.1; CV(%): 6.7 Sample 2: n=24; Mean(pg/ml): 3966; Standard deviation: 293.5; CV(%): 7.4 Sample 3: n=24; Mean(pg/ml): 7247; Standard deviation: 427.6; CV(%): 5.9

There is cross-reactivates with MMP-10 approximately 2% and no detectable cross-reactivity with other MMPs.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse MMP-3 in cell culture supernates, serum and plasma(heparin).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse MMP-3 antibody 1 ST0000-10 Lyophilized recombinant mouse MMP-3 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse MMP-3 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Matrix metalloproteinase-3(MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase(MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.

MMP3

Stromelysin-1

matrix metallopeptidase 3 (stromelysin 1, progelatinase)

CHDS6 matrix metallopeptidase 3 (stromelysin 1, progelatinase) Matrix metalloproteinase 3 Matrix metalloproteinase 3 preproprotein Matrix metalloproteinase-3 MGC126102 MGC126103 MGC126104 MMP 3 MMP-3 MMP3 MMP3_HUMAN Progelatinase Proteoglycanase SL 1 SL-1 SL1 STMY STMY1 STR1 Stromelisin 1 Stromelysin 1 Stromelysin 1 progelatinase Stromelysin-1 Transin 1 Transin-1

P28862