Boster Immunoleader

ELISA/assay

Human MMP-2 PicoKine ELISA Kit

EK0459

1 x96 well plate

380 USD

Human MMP-2 PicoKine ELISA Kit

EK0459

380 USD

Sandwich ELISA kit for Quantitative Detection of Human MMP-2

NSO, A30-C660

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 1526; Standard deviation: 74.8; CV(%): 4.9 Sample 2: n=16; Mean(pg/ml): 3864; Standard deviation: 216.4; CV(%): 5.6 Sample 3: n=16; Mean(pg/ml): 6733; Standard deviation: 444.4; CV(%): 6.6

Sample 1: n=24; Mean(pg/ml): 1705; Standard deviation: 97.2; CV(%): 5.7 Sample 2: n=24; Mean(pg/ml): 4012; Standard deviation: 256.8; CV(%): 6.4 Sample 3: n=24; Mean(pg/ml): 6928; Standard deviation: 512.7; CV(%): 7.4

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human MMP-2 in cell culture supernates, serum and plasma(heparin).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human MMP-2 antibody 1 ST0000-10 Lyophilized recombinant human MMP-2 standard 10ng/tubex2 AR1107 Biotinylated anti- human MMP-2 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. The standard product used in this kit is recombinant human MMP-2, consisting of 631 amino acids with the molecular mass of 71KDa. The detected MMP-2 includes zymogen and active enzyme.

MMP2

72 kDa type IV collagenase

matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

72 kDa gelatinase 72 kDa type IV collagenase CLG 4 CLG 4A CLG4 CLG4A Collagenase type IV A EC 3.4.24.24 Gelatinase A Gelatinase alpha Gelatinase neutrophil Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) Matrix metalloproteinase 2 Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase) Matrix metalloproteinase II Matrix metalloproteinase-2 MMP 2 MMP II MMP-2 MMP2 MMP2_HUMAN MONA Neutrophil gelatinase PEX TBE 1 TBE-1

P08253