Boster Immunoleader

ELISA/assay

Rat MIP-3 alpha/CCL20 PicoKine ELISA Kit

EK0455

1 x96 well plate

390 USD

Rat MIP-3 alpha/CCL20 PicoKine ELISA Kit

EK0455

390 USD

Sandwich ELISA kit for Quantitative Detection of Rat MIP-3 alpha/CCL20

E.coli, S27-M96

1 x96 well plate

7.8pg/ml-500pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 48; Standard deviation: 2.45; CV(%): 5.1 Sample 2: n=16; Mean(pg/ml): 145; Standard deviation: 6.67; CV(%): 4.6 Sample 3: n=16; Mean(pg/ml): 273; Standard deviation: 17.2; CV(%): 6.3

Sample 1: n=24; Mean(pg/ml): 67; Standard deviation: 4.36; CV(%): 6.5 Sample 2: n=24; Mean(pg/ml): 162; Standard deviation: 8.75; CV(%): 5.4 Sample 3: n=24; Mean(pg/ml): 294; Standard deviation: 22.05; CV(%): 7.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat MIP-3 alpha in cell culture supernates, serum and plasma(heparin, EDTA).

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat MIP-3 alpha/CCL20 antibody 1 ST0000-10 Lyophilized recombinant rat MIP-3 alpha/CCL20 standard 10ng/tubex2 AR1107 Biotinylated anti- rat MIP-3 alpha/CCL20 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

CCL20

C-C motif chemokine 20

chemokine (C-C motif) ligand 20

Beta chemokine exodus 1 Beta-chemokine exodus-1 C C motif chemokine 20 CC chemokine LARC Ccl20 CCL20(2-70) CCL20_HUMAN Chemokine (C C motif) ligand 20 CKb4 Exodus 1 LARC Liver and activation regulated chemokine Liver and activation-regulated chemokine Macrophage inflammatory protein 3 alpha MIP 3 alpha MIP 3a MIP-3-alpha MIP3 alpha MIP3A MIP3alpha SCYA20 Small inducible cytokine A20 Small inducible cytokine subfamily A (Cys Cys) member 20 Small-inducible cytokine A20 ST38

P97884