Boster Immunoleader

ELISA/assay

Mouse MDC PicoKine ELISA Kit

EK0447

1 x96 well plate

380 USD

Mouse MDC PicoKine ELISA Kit

EK0447

380 USD

Sandwich ELISA kit for Quantitative Detection of Mouse MDC

E.coli, G25-S92

1 x96 well plate

15.6pg/ml-1000pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 124; Standard deviation: 8.31; CV(%): 6.7 Sample 2: n=16; Mean(pg/ml): 377; Standard deviation: 18.5; CV(%): 4.9 Sample 3: n=16; Mean(pg/ml): 682; Standard deviation: 36.83; CV(%): 5.4

Sample 1: n=24; Mean(pg/ml): 147; Standard deviation: 10.88; CV(%): 7.4 Sample 2: n=24; Mean(pg/ml): 421; Standard deviation: 24.42; CV(%): 5.8 Sample 3: n=24; Mean(pg/ml): 714; Standard deviation: 48.55; CV(%): 6.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse MDC in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse MDC antibody 1 ST0000-10 Lyophilized recombinant mouse MDC standard 10ng/tubex2 AR1107 Biotinylated anti- mouse MDC antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Macrophage-derived chemokine(MDC), also called Chemokine, cc motif, ligand 22(CCL22) or Small inducible cytokine subfamily A, member 22(SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine(TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

CCL22

C-C motif chemokine 22

chemokine (C-C motif) ligand 22

A-152E5.1 ABCD 1 ABCD1 C-C motif chemokine 22 CC chemokine STCP-1 Ccl22 CCL22_HUMAN Chemokine (C-C motif) ligand 22 DC/B-CK Macrophage-derived chemokine MDC(1-69) MDC(7-69) MGC34554 SCYA22 Small inducible cytokine A22 Small inducible cytokine subfamily A (Cys-Cys), member 22 Small-inducible cytokine A22 STCP 1 STCP1 Stimulated T cell chemotactic protein 1 Stimulated T-cell chemotactic protein 1

O88430