Boster Immunoleader

ELISA/assay

Mouse M-CSF PicoKine ELISA Kit

EK0445

1 x96 well plate

390 USD

Mouse M-CSF PicoKine ELISA Kit

EK0445

390 USD

Sandwich ELISA kit for Quantitative Detection of Mouse M-CSF

E.coli, K33-E262

1 x96 well plate

15.6pg/ml-1000pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 92; Standard deviation: 5.06; CV(%): 5.5 Sample 2: n=16; Mean(pg/ml): 316; Standard deviation: 19.28; CV(%): 6.1 Sample 3: n=16; Mean(pg/ml): 628; Standard deviation: 40.2; CV(%): 6.4

Sample 1: n=24; Mean(pg/ml): 127; Standard deviation: 7.493; CV(%): 5.9 Sample 2: n=24; Mean(pg/ml): 439; Standard deviation: 27.66; CV(%): 6.3 Sample 3: n=24; Mean(pg/ml): 685; Standard deviation: 51.4; CV(%): 7.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse M-CSF in cell culture supernates, tissue homogenates, serum and plasma(EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse M-CSF antibody 1 ST0000-10 Lyophilized recombinant mouse M-CSF standard 10ng/tubex2 AR1107 Biotinylated anti- mouse M-CSF antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

M-CSF, also called CSF1, consists of a 14-amino acid peptide, longer than the usual 8-to-11 mer recognized by most CTLs. The M-CSF gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb1. Although it is a single-copy gene, its expression results in the synthesis of several mRNAs, ranging in size from about 1.5 to 4.5 kb2. There are 2 forms of M-CSF, with 224 and 522 amino acids, resulting from alternative splicing3. Furthermore, M-CSF has a role in development of the placenta. Uterine M-CSF concentration is regulated by a synergistic action of estradiol and progesterone. M-CSF is produced by uterine glandular epithelial cells. It had been found that FMS, the M-CSF receptor, is expressed in placenta and choriocarcinoma cell lines4.

CCL3

Macrophage colony-stimulating factor 1

chemokine (C-C motif) ligand 3

Colony stimulating factor 1 Colony stimulating factor 1 (macrophage) Colony stimulating factor macrophage specific CSF 1 CSF-1 CSF1 CSF1_HUMAN Csfm Lanimostim M CSF M-CSF Macrophage colony stimulating factor Macrophage colony stimulating factor 1 MCSF MGC31930 op OTTHUMP00000013364 OTTHUMP00000013365 OTTMUSP00000032803 OTTMUSP00000032804 Processed macrophage colony-stimulating factor 1 RP11-195M16.2

P07141