Boster Immunoleader

ELISA/assay

Human Leptin PicoKine ELISA Kit

EK0437

1 x96 well plate

300 USD

Human Leptin PicoKine ELISA Kit

EK0437

300 USD

Sandwich ELISA kit for Quantitative Detection of Human Leptin

E.coli, V22-C167

1 x96 well plate

62.5pg/ml-4000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 1026; Standard deviation: 37.96; CV(%): 3.7 Sample 2: n=16; Mean(pg/ml): 2535; Standard deviation: 81.12; CV(%): 3.2 Sample 3: n=16; Mean(pg/ml): 3542; Standard deviation: 123.97; CV(%): 3.5

Sample 1: n=24; Mean(pg/ml): 990; Standard deviation: 57.42; CV(%): 5.8 Sample 2: n=24; Mean(pg/ml): 2674; Standard deviation: 120.33; CV(%): 4.5 Sample 3: n=24; Mean(pg/ml): 3305; Standard deviation: 125.59; CV(%): 3.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Leptin in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Leptin antibody 1 ST0000-10 Lyophilized human Leptin standard 10ng/tubex2 AR1107 Biotinylated anti- human Leptin antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Leptin(or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q. The standard product used in this kit is recombinant human Leptin.

LEP

Leptin

leptin

FLJ94114 LEP LEP_HUMAN Leptin Leptin Murine Obesity Homolog Leptin Precursor Obesity Factor OB Obese protein Obesity factor Obesity homolog mouse Obesity murine homolog leptin OBS

A4D0Y8