Boster Immunoleader

ELISA/assay

Human Laminin PicoKine ELISA Kit

EK0434

1 x96 well plate

420 USD

Human Laminin PicoKine ELISA Kit

EK0434

420 USD

Sandwich ELISA kit for Quantitative Detection of Human Laminin

from human fibroblasts

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 1335; Standard deviation: 72.09; CV(%): 5.4 Sample 2: n=16; Mean(pg/ml): 4334; Standard deviation: 286; CV(%): 6.6 Sample 3: n=16; Mean(pg/ml): 7066; Standard deviation: 296.8; CV(%): 4.2

Sample 1: n=24; Mean(pg/ml): 1728; Standard deviation: 110.6; CV(%): 6.4 Sample 2: n=24; Mean(pg/ml): 4566; Standard deviation: 342.5; CV(%): 7.5 Sample 3: n=24; Mean(pg/ml): 7324; Standard deviation: 366.2; CV(%): 5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Laminin in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Laminin antibody 1 ST0000-10 Lyophilized human Laminin standard 10ng/tubex2 AR1107 Biotinylated anti- human Laminin antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31. The standard product used in this kit is isolated from plasma.

LAMA1; LAMA2; LAMB1; LAMB2

Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2

laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2(laminin S)

Lamb2 LAMB2_HUMAN LAMC1 LAMC2 LAMC3 laminin A Laminin B Laminin B1s chain Laminin gamma 1 Laminin gamma 2 Laminin gamma 3 Laminin subunit beta-2 Laminin-11 subunit beta Laminin-14 subunit beta Laminin-15 subunit beta Laminin-3 subunit beta Laminin-4 subunit beta Laminin-7 subunit beta Laminin-9 subunit beta OCCM S-LAM beta S-laminin subunit beta

B0YJ33