Boster Immunoleader

ELISA/assay

Human IL-12(p40) PicoKine ELISA Kit

EK0423

1 x96 well plate

250 USD

Human IL-12(p40) PicoKine ELISA Kit

EK0423

250 USD

Sandwich ELISA kit for Quantitative Detection of Human IL-12(p40)

sf21, I23-S328

1 x96 well plate

31.2pg/ml-2000pg/ml

<2pg/ml

Sample 1: n=16; Mean(pg/ml): 276; Standard deviation: 16.56; CV(%): 6 Sample 2: n=16; Mean(pg/ml): 772; Standard deviation: 41.7; CV(%): 5.4 Sample 3: n=16; Mean(pg/ml): 1230; Standard deviation: 59.04; CV(%): 4.8

Sample 1: n=24; Mean(pg/ml): 316; Standard deviation: 23.07; CV(%): 7.3 Sample 2: n=24; Mean(pg/ml): 869; Standard deviation: 56.5; CV(%): 6.5 Sample 3: n=24; Mean(pg/ml): 1475; Standard deviation: 78.2; CV(%): 5.3

There is cross-reactivates with IL-12(p70), IL-23(p19/p40)< 5%.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human IL-12(p40) in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human IL-12(P40) antibody 1 ST0000-10 Lyophilized recombinant human IL-12(P40) standard 10ng/tubex2 AR1107 Biotinylated anti- human IL-12(P40) antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Interleukin-12(IL12; formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits. The larger 40-kDa subunit(p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit(p35) is related to IL6 and GCSF. Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer. Interleukin(IL)-12 has been cloned on the basis of its ability to activate natural killer(NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon(IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype. The subunits map to different chromosomes: p40(IL12B) to 5q31-q33 and p35(IL12A) to 3p12-3q13.2.2 The standard product used in this kit is recombinant human IL-12(p40), which is a 40KDa glycoprotein consisting of 306 amino acids.

IL12B

Interleukin-12 subunit beta

interleukin 12B

40 kD Subunit CLMF CLMF 2 CLMF p40 CLMF2 Cytotoxic lymphocyte maturation factor 2 Cytotoxic lymphocyte maturation factor 2 p40 Cytotoxic lymphocyte maturation factor 40 kDa subunit HGNC:5970 IL 12 subunit p40 IL 12B IL-12 subunit p40 IL-12B IL12 subunit p40 IL12B IL12B_HUMAN Interleukin 12 beta chain Interleukin 12 p40 Interleukin 12 subunit beta Interleukin 12B Interleukin-12 subunit beta Interleukin12B Natural killer cell stimulatory factor 2 Natural killer cell stimulatory factor 40 kD subunit NK cell stimulatory factor chain 2 NKSF NKSF 2 NKSF2 p40 Subunit P40 T cell stimulating factor TcMF TSF

P29460