Boster Immunoleader

ELISA/assay

Mouse IL-12(p70) PicoKine ELISA Kit

EK0422

1 x96 well plate

350 USD

Mouse IL-12(p70) PicoKine ELISA Kit

EK0422

350 USD

Sandwich ELISA kit for Quantitative Detection of Mouse IL-12(p70)

sf21, (M23-S335)+(R23-A215)

1 x96 well plate

15.6pg/ml-1000pg/ml

<1.5pg/ml

Sample 1: n=16; Mean(pg/ml): 97; Standard deviation: 6.5; CV(%): 6.7 Sample 2: n=16; Mean(pg/ml): 367; Standard deviation: 25.7; CV(%): 7 Sample 3: n=16; Mean(pg/ml): 633; Standard deviation: 34.2; CV(%): 5.4

Sample 1: n=24; Mean(pg/ml): 123; Standard deviation: 9.23; CV(%): 7.5 Sample 2: n=24; Mean(pg/ml): 408; Standard deviation: 33.05; CV(%): 8.1 Sample 3: n=24; Mean(pg/ml): 657; Standard deviation: 38.8; CV(%): 5.9

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse IL-12(p70) in cell culture supernates and serum.

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse IL-12(p70) antibody 1 ST0000-10 Lyophilized recombinant mouse IL-12(p70) standard 10ng/tubex2 AR1107 Biotinylated anti- mouse IL-12(p70) antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

IL12A

Interleukin-12 subunit alpha

interleukin 12A

CLMF CLMF p35 Cytotoxic lymphocyte maturation factor 1 Cytotoxic lymphocyte maturation factor 1 p35 Cytotoxic lymphocyte maturation factor 35 kDa subunit IL-12 subunit p35 IL-12A IL12A IL12A_HUMAN IL35 subunit Interleukin 12 alpha chain Interleukin 12 p35 Interleukin 12 p70 Interleukin-12 subunit alpha Natural killer cell stimulatory factor 1 Natural killer cell stimulatory factor 1 35 kD subunit NF cell stimulatory factor chain 1 NFSK NK cell stimulatory factor chain 1 NKSF1 P35

F8WI71