Boster Immunoleader

ELISA/assay

Human IL-12(p70) PicoKine ELISA Kit

EK0421

1 x96 well plate

250 USD

Human IL-12(p70) PicoKine ELISA Kit

EK0421

250 USD

Sandwich ELISA kit for Quantitative Detection of Human IL-12(p70)

sf21, (I23-S328)+(R23-S219)

1 x96 well plate

7.8pg/ml-500pg/ml

<2pg/ml

Sample 1: n=16; Mean(pg/ml): 44; Standard deviation: 2.16; CV(%): 4.9 Sample 2: n=16; Mean(pg/ml): 127; Standard deviation: 7.24; CV(%): 5.7 Sample 3: n=16; Mean(pg/ml): 283; Standard deviation: 17.83; CV(%): 6.3

Sample 1: n=24; Mean(pg/ml): 67; Standard deviation: 3.89; CV(%): 5.8 Sample 2: n=24; Mean(pg/ml): 158; Standard deviation: 9.48; CV(%): 6 Sample 3: n=24; Mean(pg/ml): 322; Standard deviation: 23.2; CV(%): 7.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human IL-12(p70) in cell culture supernates and serum.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human IL-12(P70) antibody 1 ST0000-10 Lyophilized recombinant human IL-12(P70) standard 10ng/tubex2 AR1107 Biotinylated anti- human IL-12(P70) antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response.[i] And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.[ii] Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.[iii] The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.

IL12A

Interleukin-12 subunit alpha

interleukin 12A

CLMF CLMF p35 Cytotoxic lymphocyte maturation factor 1 Cytotoxic lymphocyte maturation factor 1 p35 Cytotoxic lymphocyte maturation factor 35 kDa subunit IL-12 subunit p35 IL-12A IL12A IL12A_HUMAN IL35 subunit Interleukin 12 alpha chain Interleukin 12 p35 Interleukin 12 p70 Interleukin-12 subunit alpha Natural killer cell stimulatory factor 1 Natural killer cell stimulatory factor 1 35 kD subunit NF cell stimulatory factor chain 1 NFSK NK cell stimulatory factor chain 1 NKSF1 P35

O60595