Boster Immunoleader

ELISA/assay

Mouse IL-10 PicoKine ELISA Kit

EK0417

1 x96 well plate

350 USD

Mouse IL-10 PicoKine ELISA Kit

EK0417

350 USD

Sandwich ELISA kit for Quantitative Detection of Mouse IL-10

E.coli, S19-S178

1 x96 well plate

15.6pg/ml-1000pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 82; Standard deviation: 3.77; CV(%): 4.6 Sample 2: n=16; Mean(pg/ml): 413; Standard deviation: 21.5; CV(%): 5.2 Sample 3: n=16; Mean(pg/ml): 724; Standard deviation: 44.2; CV(%): 6.1

Sample 1: n=24; Mean(pg/ml): 113; Standard deviation: 7; CV(%): 6.2 Sample 2: n=24; Mean(pg/ml): 367; Standard deviation: 24.96; CV(%): 6.8 Sample 3: n=24; Mean(pg/ml): 628; Standard deviation: 46.5; CV(%): 7.4

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse IL-10 in cell culture supernates and serum.

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse IL-10 antibody 1 ST0000-10 Lyophilized recombinant mouse IL-10 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse IL-10 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant mouse IL-10, consisting of 160 amino acid with the molecular mass of 18KDa.

IL10

Interleukin-10

interleukin 10

CSIF Cytokine synthesis inhibitory factor GVHDS IL 10 IL-10 IL10 IL10_HUMAN IL10A Interleukin 10 Interleukin-10 Interleukin10 MGC126450 MGC126451 T cell growth inhibitory factor TGIF

P18893